PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Vieira D.S., Rodrigues T.C.S., de Miranda R.L., Cardoso G.F., Guimarães E.C., Brites V.L.C. & Mundim A.V. 2014. [Determination of proteins and metabolites concentrations in plasma of rattlesnakes in captivity.] Determinação das concentrações plasmáticas de proteínas e metabólitos de cascavéis em cativeiro. Pesquisa Veterinária Brasileira 34(Supl.1):39-42. Laboratório Clínico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Av. Mato Grosso 3289, Uberlândia, MG 38405-314, Brasil. E-mail: danivbio@gmail.com For determining plasma concentrations of proteins and metabolites of rattlesnakes in captivity, 60 adult snakes, 30 males and 30 females were used. Blood was collected by puncture of the cervical paravertebral venous sinus and stored in tubes with heparin. Biochemical analyzes were colorimetrically processed using an Automatic Biochemistry Analyzer Chemwell (Awareness Technology®, Inc). The mean and standard deviation were calculated for the following constituents: total protein, albumin, globulin, albumin/globulin ratio, uric acid, creatinine, urea, cholesterol, HDL cholesterol and triglycerides. The values were similar to those previously reported for reptiles and snakes, with the differences observed probably due to the difference between species, climate, season and the methodology used. There were no significant differences between males and females for the parameters studied. These results may be useful in establishing normal biochemical values for conservation plans for these snakes in captivity.

• Blood biochemistry Crotalus durissus rattle snake bioquímica sanguínea cascavel

Abstract in Portuguese:

RESUMO.- Vieira D.S., Rodrigues T.C.S., de Miranda R.L., Cardoso G.F., Guimarães E.C., Brites V.L.C. & Mundim A.V. 2014. [Determination of proteins and metabolites concentrations in plasma of rattlesnakes in captivity.] Determinação das concentrações plasmáticas de proteínas e metabólitos de cascavéis em cativeiro. Pesquisa Veterinária Brasileira 34(Supl.1):39-42. Laboratório Clínico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Av. Mato Grosso 3289, Uberlândia, MG 38405-314, Brasil. E-mail: danivbio@gmail.com Para determinar as concentrações plasmáticas de proteínas e metabólitos de cascavéis em cativeiro, foram utilizadas 60 serpentes adultas, sendo 30 machos e 30 fêmeas. O sangue foi coletado através de punção do seio venoso paravertebral cervical e armazenado em tubos com heparina. As análises bioquímicas foram processadas colorimetricamente em Analisador Automático de Bioquímica Chemwell (Awareness Technology®, Inc). Foram calculadas as médias e desvios padrão dos seguintes constituintes: proteínas totais, albumina, globulinas, relação albumina/globulinas, ácido úrico, creatinina, ureia, colesterol, colesterol HDL e triglicérides. Os valores obtidos foram semelhantes aos descritos na literatura para repteis e serpentes, sendo as diferenças observadas provavelmente decorrentes da diferença entre espécies, clima, estação do ano e metodologia utilizada. Não houve diferenças significativas entre machos e fêmeas para os parâmetros estudados. Estes resultados podem ser úteis no estabelecimento de valores de referência para planos de conservação destes ofídios em cativeiro.

Abstract in English:

ABSTRACT.- Miranda R.L., Mundim A.V., Saquy A.C.S, Costa A.S., Guimarães E.C., Gonçalves F.C. & Silva F.O.C. 2011. [Hematologic profile of horses subjected to Team Penning.] Perfil hematológico de equinos submetidos à prova de Team Penning. Pesquisa Veterinária Brasileira 31(1):81-86. Laboratório Clínico e de Anatomia Animal, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Av. Pará 1720, Bloco 2T, Campus Umuarama, Uberlândia, MG 38400-902, Brazil. E-mail: renatavetufu@yahoo.com.br Variations in hematologic parameters are used to assess the degree of training or clinical state of the animal. The hematologic evaluation of horses at rest has been an object of study in order to establish a correlation with training or athletic capacity. The purpose of this study was to evaluate the hematologic profile of horses subjected to Team Penning competitions, correlating sex and frequency of physical activity. Two milliliters of blood were drawn through a puncture made in the external jugular vein from 29 horses, 18 males and 11 females, at rest (Moment I) and after exercising (Moment II). The blood samples were processed in an ABC VET automated veterinary hematology analyzer (Horiba ABX Diagnostics). The animals were divided into Group A, B, C and D according to the number of times they participated in the competition. The values of globular volume, hemoglobin, erythrocytes, rod-shaped segmented neutrophils and monocytes increased after the physical exercise, unlike the number of lymphocytes and eosinophils, which decreased. A comparison of the before/after exercise correlations showed no significant differences (p<0.05) between males and females. In addition, it was found that the value of the MI/MII ratio for the globular volume, hemoglobin and number of erythrocytes varied according to the frequency of the exercise. It was concluded that the Team Penning competition produces hematologic alterations in horses, which are affected by the frequency of exercising, regardless of sex.

• Team Penning Equus caballus

Abstract in Portuguese:

RESUMO.- Miranda R.L., Mundim A.V., Saquy A.C.S, Costa A.S., Guimarães E.C., Gonçalves F.C. & Silva F.O.C. 2011. [Hematologic profile of horses subjected to Team Penning.] Perfil hematológico de equinos submetidos à prova de Team Penning. Pesquisa Veterinária Brasileira 31(1):81-86. Laboratório Clínico e de Anatomia Animal, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Av. Pará 1720, Bloco 2T, Campus Umuarama, Uberlândia, MG 38400-902, Brazil. E-mail: renatavetufu@yahoo.com.br

Abstract in English:

ABSTRACT.- Miranda R.L., Mundim A.V., Costa À.S., Guimarães R.V. & Silva F.O.C. 2008. Serum biochemistry of 4-day-old ostriches (Struthio camelus). Pesquisa Veterinária Brasileira 28(9):423-426. Laboratório Clínico Veterinário da Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Av. Pará 1720, Bloco 2T, Campus Umuarama, Uberlândia, MG 38400-902, Brazil. E-mail: renatavetufu@yahoo.com.br An analysis was made of 30 four-day-old ostriches to evaluate their protein, metabolite, mineral, and serum enzyme profiles, to correlate them with the birds’ sex. The values obtained were: Total proteins 3.59±0.72g/dL, albumin 1.04±0.14g/dL, globulins 2.51±0.56g/dL, A:G ratio 0.43± 0.07, total cholesterol 615.10±101.15mg/dL, high density lipoprotein cholesterol (HDL-C) 132.72±20.33mg/dL, low density lipoprotein cholesterol (LDL-C) 454.93±90.81mg/dL, very low density lipoprotein cholesterol (VLDL-C) 27.45±9.96mg/dL, triglycerides 137.23±49.78mg/dL, uric acid 6.24±2.15mg/dL, urea 18.27±12.33mg/dL, creatinine 0.30±0.04mg/dL, total calcium 9.38± 0.76mg/dL, ionized calcium 7.17±0.64mg/dL, phosphorus 6.96±0.91mg/dL, Ca:P ratio 1.37±0.21, iron 24.74±13.02µg/dL, sodium 142.03±6.17mEq/L, chlorides 109.59± 4.99mEq/L, aspartate aminotransferase (AST) 200.67±31.42 U/L, alanine aminotransferase (ALT) 3.90±1.92 U/L, g-glutamyltransferase (GGT) 1.18±0.73 U/L, alkaline phosphatase (ALP) 597.30± 231.36 U/L, and creatine kinase (CK) 2348.30±755.60 U/L. Males and females showed significant differences in total proteins, globulins, alkaline phosphatase, A:G ratio, and uric acid.

• Serum biochemistry ostriches Struthio camelus

Abstract in Portuguese:

ABSTRACT.- Miranda R.L., Mundim A.V., Costa À.S., Guimarães R.V. & Silva F.O.C. 2008. Serum biochemistry of 4-day-old ostriches (Struthio camelus). Pesquisa Veterinária Brasileira 28(9):423-426. Laboratório Clínico Veterinário da Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Av. Pará 1720, Bloco 2T, Campus Umuarama, Uberlândia, MG 38400-902, Brazil. E-mail: renatavetufu@yahoo.com.br An analysis was made of 30 four-day-old ostriches to evaluate their protein, metabolite, mineral, and serum enzyme profiles, to correlate them with the birds’ sex. The values obtained were: Total proteins 3.59±0.72g/dL, albumin 1.04±0.14g/dL, globulins 2.51±0.56g/dL, A:G ratio 0.43± 0.07, total cholesterol 615.10±101.15mg/dL, high density lipoprotein cholesterol (HDL-C) 132.72±20.33mg/dL, low density lipoprotein cholesterol (LDL-C) 454.93±90.81mg/dL, very low density lipoprotein cholesterol (VLDL-C) 27.45±9.96mg/dL, triglycerides 137.23±49.78mg/dL, uric acid 6.24±2.15mg/dL, urea 18.27±12.33mg/dL, creatinine 0.30±0.04mg/dL, total calcium 9.38± 0.76mg/dL, ionized calcium 7.17±0.64mg/dL, phosphorus 6.96±0.91mg/dL, Ca:P ratio 1.37±0.21, iron 24.74±13.02µg/dL, sodium 142.03±6.17mEq/L, chlorides 109.59± 4.99mEq/L, aspartate aminotransferase (AST) 200.67±31.42 U/L, alanine aminotransferase (ALT) 3.90±1.92 U/L, g-glutamyltransferase (GGT) 1.18±0.73 U/L, alkaline phosphatase (ALP) 597.30± 231.36 U/L, and creatine kinase (CK) 2348.30±755.60 U/L. Males and females showed significant differences in total proteins, globulins, alkaline phosphatase, A:G ratio, and uric acid.